Cataloguenumber
CYT-1010
Synonyms
Introduction
Description
SomatotropinRainbowTrout(Oncorhynchusmykiss)RecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining188aminoacidswithanadditionalAlaattheN-terminusandhavingamolecularmassof21,535Dalton.
TheRainbowTrout(Oncorhynchusmykiss)Growth-HormoneRecombinantispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.5%NaHCO3.AdjustedtopH-8.
Solubility
ItisrecommendedtoreconstitutethelyophilizedGrowth-HormoneRainbowTrout(Oncorhynchusmykiss)in0.4%NaHCO3orwateradjustedtopH8-9,notlessthan100µg/mlandnotmorethan3mg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions,preferablyinapresenceofacarrierproteinsuchasBSAorsimilar.
StABIlity
LyophilizedGrowth-HormoneRainbowTrout(Oncorhynchusmykiss)althoughstableatroomtemperatureforatleasttwoweeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionandfiltersterilizationGHcanbestoredat4°C,pH9forupto4weeks.Forlongtermstorageandmoredilutedsolutionsitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:
(a)AnalysisbySEC-HPLC.
(b)AnalysisbySDS-PAGE.
Aminoacidsequence
AIENQRLFNIAVSRVQHLHLLAQKMFNDFDGTLLPDERRQLNKIFLLDFCNSDSIVSPVD
KHETQKSSVLKLLHISFRLIESWEYPSQTLIISNSLMVRNANQISEKLSDLKVGINLLIT
GSQDGVLSLDDNDSQQLPPYGNYYQNLGGDGNVRRNYELLACFKKDMHKVETYLTVAKCR
KSLEANCTL.
BIOLOGicalActivity
SomatotropinRainbowTrout(Oncorhynchusmykiss)RecombinantisbiologicallyactiveinPDF-P13B9cellsstabletransfectedwithrabbitGHreceptors,thoughitsactivityisabout10foldlowerthanthatofhumanGH.