Cataloguenumber
CYT-1000
Synonyms
Milkfatglobule-EGFfactor8protein,isoformCRA_a,Putativeuncharacterizedprotein,Mfge8,mCG_6301.
Introduction
Milkfatglobule-EGFfactor8protein(Mfge8)ispleiotropicsecretedglycoproteinwhichpromotesmammaryglandmorphogenesis,angiogenesis,andtumorprogression.Mfge8hasalsoanimperativeroleintissuehomeostasisandthepreventionofinflammation.Mfge8functionsasabridgebetweenphosphatidylserineonapoptoticcellsandIntegrinalphaVbeta3onphagocytes,leADIngtotheclearanceofapoptoticdebris.
Description
MFGE8MouseRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining413aminoacids(23-426a.a.)andhavingamolecularmassof46kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).
MFGE8isexpressedwitha6aminoacidHis-tagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
MFGE8proteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADLASGDFCDSSLCLNGGTCLTGQDNDIYCLCPEGFTGLVCNETERGPCSPNPCYNDAKCLVTLDTQRGDIFTEYICQCPVGYSGIHCETGCSTQLGMEGGAIADSQISASSVYMGFMGLQRWGPELARLYRTGIVNAWTASNYDSKPWIQVNLLRKMRVSGVMTQGASRAGRAEYLKTFKVAYSLDGRKFEFIQDESGGDKEFLGNLDNNSLKVNMFNPTLEAQYIKLYPVSCHRGCTLRFELLGCELHGCSEPLGLKNNTIPDSQMSASSSYKTWNLRAFGWYPHLGRLDNQGKINAWTAQSNSAKEWLQVDLGTQRQVTGIITQGARDFGHIQYVASYKVAHSDDGVQWTVYEEQGSSKVFQGNLDNNSHKKNIFEKPFMARYVRVLPVSWHNRITLRLELLGCHHHHHH.