Cataloguenumber
CYT-1015
Synonyms
Macrophagecolony-stimulatingfactor1,CSF-1,M-CSF,MCSF,Lanimostim.
Introduction
Granulocyte/MacrophageColony-StimulatingFactorsarecytokinesthatactinhematopoiesisbycontrollingtheproduction,differentiation,andfunctionof2relatedwhitecellpopulationsoftheblood,thegranulocytesandthemonocytes-macrophages.MCSFinducescellsofthemonocyte/macrophagelineage.MCSFplaysaroleinimmunologicaldefenses,bonemetabolism,lipoproteinsclearance,fertilityandpregnancy.
Description
MCSFproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining231aminoacids(33-255a.a.)andhavingamolecularmassof26.1kDa(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).
MCSFisexpressedwithan8aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
MCSFproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
MeasuredinacellproliferationassayusingM-NFS-60mousemyelogenousleukemialymphoblastcell.TheED50forthiseffectislessorequalto3ng/ml.
Aminoacidsequence
EEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQDVVTKPDCNCLYPKAIPSSDPASVSPHQPLAPSMAPVAGLTWEDSEGTEGSSLLPGEQPLHTVDPGSAKQRPPRLEHHHHHH.