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Prospec/CTGF Antibody/Mouse Anti Human Connective Tissue Growth Factor/20µg/ANT-699-20µg
Cataloguenumber
ANT-699
Synonyms
CCN2,NOV2,HCS24,IGFBP8,MGC102839,CTGF,ConnectiveTissueGrowthFactor.
Type
Introduction
ConnectiveTissueGrowthFactorbelongstotheCCNfamilyofproteins.TheCCNfamilypresentlyconsistsofsixmembersinhumanalsoknownas:Cyr61(Cysteinrich61),CTGF(ConnectiveTissueGrowthFactor),Nov(NephroblastomaOverexpressedgene),WISP-1,2and3(Wnt-1InducedSecretedProteins).TheCCNgenesencodesecretedproteinsassociatedwiththeExtracellularMatrix(ECM)andcellmembrane.CCNproteinsarematricellularproteinswhichareinvolvedintheregulationofvariouscellularfunctionsincluding:proliferation,differentiation,survival,adhesionandmigration.Theyareexpressedinderivativesofthethreeembryonicsheetsandareimplicatedinthedevelopmentofkidney,nervoussystem,muscle,bonemarrow,cartilageandbone.Duringadulthood,theyareimplicatedinwoundhealing,bonefracturerepair,andpathologiessuchas:fibrosis,vascularailmentsandtumOrigenesis.FulllengthsecretedCCNproteinscanshowanantiproliferativeactivity,whereastruncatedisoformsarelikelytostimulateproliferationandbehaveasoncogenes.ThefulllengthproteinconsistsoffourmodulesModuleIsharespartialidentitywiththeN-terminalpartoftheInsulin-likeGrowthFactorBindingProteins(IGFBPs).ModuleIIincludesastretchof70aminoacidresidues–whichsharessequenceidentitywiththeVonWillebrandFactorTypeCrepeat(VWC).ModuleIIIcontainssequencessharingidentitywiththeThrombospondintype1repeat(TSP1)(WSXCSXXCG),whichisthoughttobeimplicatedinthebindingofsulfatedglycoconjugatesandtobeimportantforcelladhesion.ModuleIV,alsodesignatedCT,isencodedbyexon5.Itistheleastsconservedoneofthefourdomainsatthelevelofnucleotidesequence,butitappearstobecriticalforseveraloftheBIOLOGicalfunctionsattributedtotheCCNproteins.ModuleIVresemblestheCTdomainofseveralextracellularproteinincluding,VonWillebrand"sfactorandmucins.Sequencesimilaritiestoheparin-bindingmotifsarealsofoundwithinthisdomain.Proteolysisofthesecretedfull-lengthCCNproteinsthathasbeenreportedinthecaseofCCN2andCCN3mightresultintheproductionofCCN-derivedpeptideswithhighaffinityforligandsthatfull-lengthCNNproteinsbindonlypoorly.Amino-truncatedCCN2isoformswerebiologicallyactivewhereasnospecificbiologicalactivityhasbeenattributedtothetruncatedCCN3.Althoughthemolecularprocessesunderlyingtheproductionofthesesecretedisoformsispresentlyunknown,itisimportanttonotethatproteolysisoccuratthesameaminoacidresiduesinbothCCN2andCCN3.AnelevatedexpressionofCCN2hasalsobeendetectedbyNorthernblottinginhumaninvasivemammaryductalcarcinomas,dermatofibromas,pyogenicgranuloma,endothelialcellsofangiolipomasandangioleiomyomas,andinpancreatictumors.AstudyperformedwithchondrosarcomasrepresentativeofvarioushistologicalgradesestablishedthatCCN2expressionwascloselycorrelatedwithincreasinglevelsofmalignancy.InagreementwithCCN2playingaroleinbraintumorangiogenesis,immunocytochemistrystudiesindicatedthatbothglioblastomatumorcellsandproliferatingendothelialcellsstainedpositiveforCCN2.Inastrocytomas,CCN2expressionwasparticularlyelevatedinhighgradetumors,withamarkedeffectofCCN2oncellproliferation.DownregulationofCCN2expressioninthesecellswasassociatedwithagrowtharrestattheG1/Stransitionwhileover-expressionofCCN2inducedatwo-foldincreaseofthenumberofcellsintheG1phase.Geneprofilinganalysisallowedtoidentifyasetofabout50geneswhoseexpressionmightaccountfortheproliferativeactivityofCCN2inthesecells.CCN2wasseeninahigherproportionofmononuclearcellsofpatientswithacutelymphoblasticleukemia.
Clone
PAT18E7AT.
PhysicalAppearance
Immunogen
Anti-humanCTGFmAb,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwitharecombinanthumanCTGFprotein27-349aminoacidspurifiedfromE.coli.
IgSubclass
MouseIgG2aheavychainandκlightchain.
PurificationMethod
CTGFantibodywaspurifiedbyprotein-Aaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
StorageProcedures
StABIlity/ShelfLife
Applications
CTGFantibodyhasbeentestedbyELISA,WesternblotanalysisandICC/IFtoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.
SafetyDataSheet
SDS
