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Prospec/Fribronectin Recombinant/Fibronectin Human Recombinant/20µg/PRO-2621-20µg

Cataloguenumber
PRO-2621
Introduction
Fibronectintakespartinseveralcellularprocesses,includingtissuerepair,embryogenesis,bloodclotting,andcellmigration/adhesion.Plasmafibronectinleveliselevatedinseverecoronaryarterydisease.Increasedplasmafibronectinlevelsarerelatedwithvenousthromboembolism(VTE)particularlyinmales,andextendtheprobableassociationbetweenbioMarkersandriskfactorsforarterialatherothrombosisandVTE.Fibronectinconsistsin2mainforms:1)asaninsolubleglycoproteindimerthatservesasalinkerintheetracellularmatrixand2)asasolubledisulphidelinkeddimerfoundintheplasma.Theplasmaformisproducedbyhepatocytes,andtheECMformissynthesizedbyfibroblasts,chondrocytes,endothelialcells,macrophages,aswellascertainepithelialcells.Fibronectinalostakespartasageneralcelladhesionmoleculebyanchoringcellstocollagenorproteoglycansubstrates.FibronectinorganizescellularinteractionwiththeECMbybindingtodifferentcomponentsoftheextracellularmatrixandtomembrane-boundFibronectinreceptorsoncellsurfaces.
Description
FribronectinHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining574aminoacidsandhavingamolecularmassof62.6kDa.TheFribronectinispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutionin20mMTris-HCl,pH8.0,150mMNaCl,with5%Trehaloseand0.02%Tween-20.
Solubility
ItisrecommendedtoreconstitutethelyophilizedFribronectininsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
StABIlity
LyophilizedFribronectinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFribronectinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
SafetyDataSheet
SDS