Cataloguenumber
PRO-2597
Synonyms
Introduction
Avidinisatetramericproteinof4identicalsubunits(homotetramer)whichcanbindtobiotinwithahighdegreeofaffinityandspecificity.TheestimatedmolecularweightofAvidininitstetramericformisbetween66-69kDa.Avidinisproducedintheoviductsofbirds,reptilesandamphibiansandissubsequentlydepositedinthewhitesoftheireggs.Inthechickeneggwhite,avidinmakesuproughly0.05%oftotalprotein(approximately1.8mgperegg).10%ofAvidin’smolecularweightisascribedtocarbohydratecontentwhichiscomposedof4-5mannoseand3N-acetylglucosamineresidues.Avidinhasatleastthreedistinctiveoligosaccharidestructuraltypewhicharesimilarinstructureandcomposition.Thedissociationconstant(KD)ofavidinisapproximately10-15M,makingitoneofthestrongestknownnon-covalentbonds.
Description
RecombinantAvidinproducedinPlantsisapolypeptidechainhavingamolecularmassof66kDaand16kdapersubunit.
TheRecombinantAvidinispurifiedbyaffinitychromatographictechniques.
Source
Corn(ZeaMays).
PhysicalAppearance
SterileFilteredwhitelyophilizedpowder.
Solubility
ItisrecommendedtoreconstitutethelyophilizedRecombinantAvidininsterile18MΩ-cmH2Onotlessthan100µg/mlormorethan10mg/mlsolutions.
StABIlity
LyophilizedRecombinantAvidinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionRecombinantAvidinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan90%asvisualizedbySDS-PAGE.
BIOLOGicalActivity
13.5units/mgprotein,1unitbinds1µgbiotin.
SafetyDataSheet
SDS