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Other_recombinant_protein >
Prospec/L-Selectin Human, Sf9/L-Selectin Human Recombinant, Sf9/10µg/PRO-2487-10µg

Cataloguenumber
PRO-2487
Synonyms
SelectinL,Leukocyte-EndothelialCellAdhesionMolecule1,CD62Antigen-LikeFamilyMemberL,LeukocyteSurfaceAntigenLeu-8,LymphocyteAdhesionMolecule1,LymphNodeHomingReceptor,Gp90-MEL,LECAM1,LYAM1,LNHR,TQ1,LeukocyteAdhesionMolecule,PlnHomingReceptor,CD62LAntigen,L-Selectin,CD62L,PLNHR,LAM-1,LAM1,LEU8,LSEL,L-selectin.
Introduction
L-Selectinbelongstoafamilyofdivalentcation-dependentcarbohydrate-bindingglycoproteinsoradhesionmolecules.TheL-Selectinmoleculeiscomposedofvariousdomains:onehomologoustolectins,onetoepidermalgrowthfactor,andtwototheconsensusrepeatunitsfoundinC3/C4bindingproteins.L-selectinisexpressedconstitutivelyonlymphocytes,monocytesandgranulocytesandinteractsspecificallywithcarbohydrategroupsonactivatedendothelialcells.L-SelectinmaybeshedbyproteolyticcleavageandcirculatinglevelsinBIOLOGicalfluidsmaybeusedasanindicatorofvariouspathologicalconditions.L-SelectiniscleavedbyADAM17.L-selectinworksasa"homingreceptor"forleukocytestoentersecondarylymphoidtissuesviathehighendothelialvenules.LigandspresentonendothelialcellswillattachtoleukocytesexpressingL-selectin,whichcausestheleukocytestobecomelocalizedatthatjuncture.Thereceptorisalsolocatedonthecellsurfacesof"naive"Tcells,whichhavenotyetencounteredtheirparticularantigen.Thissurfaceexpressionislostfollowingthecellsactivation.
Description
L-SelectinproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining303aminoacids(52-345a.a.)andhavingamolecularmassof34.1kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).L-Selectinisexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
L-Selectinproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPWTYHYSEKPMNWQRARRFCRDNYTDLVAIQNKAEIEYLEKTLPFSRSYYWIGIRKIGGIWTWVGTNKSLTEEAENWGDGEPNNKKNKEDCVEIYIKRNKDAGKWNDDACHKLKAALCYTASCQPWSCSGHGECVEIINNYTCNCDVGYYGPQCQFVIQCEPLEAPELGTMDCTHPLGNFSFSSQCAFSCSEGTNLTGIEETTCGPFGNWSSPEPTCQVIQCEPLSAPDLGIMNCSHPLASFSFTSACTFICSEGTELIGKKKTICESSGIWSNPSPICQKLDKSFSMIKEGDYNHHHHHH.
SafetyDataSheet
SDS