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Other_recombinant_protein >
Prospec/VTN Human, Sf9/Vitronectin Human Recombinant, Sf9/20µg/pro-2594-20µg

Cataloguenumber
pro-2594
Synonyms
VN,S-protein,Serum-spreADIngfactor,V75,VTN.
Introduction
Vitronectin(VTN)whichisapartofthepexinfamilyisacelladhesionandspreadingfactorfoundinserumandtissues.VTNinteractswithglycosaminoglycansandproteoglycans.VTNinhibitsthemembrane-damagingeffectoftheterminalcytolyticcomplementpathwayandbindstonumerousserpinserineproteaseinhibitors.ScientistshavebeennoticedanoverexpressionofVTN,integrinsandplasminogeninmigratingcellsduringwoundhealing.
Description
VTNproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining468aminoacids(20-478a.a.)andhavingamolecularmassof53.3kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).VTNisexpressedwithan9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Formulation
VTNproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
BIOLOGicalActivity
MeasuredbytheabilityoftheimmobilizedproteintosupporttheadhesionofB16-F10mousemelanomacells.TheED50is5ug/mlwhencellsareaddedtoVTNcoatedplates.
Aminoacidsequence
ADPDQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAMAGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHLHHHHHH.
SafetyDataSheet
SDS