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Other_recombinant_protein >
Prospec/SNIP1 Human/Smad Nuclear Interacting Protein 1 Human Recombinant/20µg/PRO-201-20µg
Cataloguenumber
PRO-201
Synonyms
Smadnuclear-interactingprotein1,FHAdomain-containingproteinSNIP1,SNIP1,FLJ12553,dJ423B22.2,RP3-423B22.3.
Introduction
SNIP1issmadnuclearinteractingproteinthatcontainsaforkhead-associated(FHA)domainandactsasanuclearinhibitorofCBP/p300.SNIP1isaninhibitoroftheTGF-betasignaltransductionpathwayandissignificantinsuppressingtranscriptionalactivationdependentontheco-activatorsCBPandp300.InhibitionofNF-kappaBactivityisafunctionoftheN-terminaldomainofSNIP1andinvolvescompetitionofSNIP1andtheNF-kappaBsubunit,RelA/p65,forbindingtop300,similartothemechanismofinhibitionofSmadsignalingbySNIP1.
Description
SNIP1HumanRecombinantfusedwitha21aminoacidHistagatN-terminusproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining160aminoacids(258-396a.a.)andhavingamolecularmassof18.8kDa.TheSNIP1ispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheSNIP1solution(1mg/ml)contains20mMTris-HClbuffer(pH8.0),2mMDTT,20%glyceroland100mMNaCl.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMRWRLYPFKNDEVLPVMYIHRQSAYLLGRHRRIADIPIDHPSCSKQHAVFQYRLVEYTRADGTVGRRVKPYIIDLGSGNGTFLNNKRIEPQRYYELKEKDVLKFGFSSREYVLLHESSDTSEIDRKDDEDEEEEEEVSDS.
SafetyDataSheet
SDS

