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Prospec/SIT1 Human/Signaling Threshold Regulating Transmembrane Adaptor 1 Human Recombinant/5µg/PRO-928-5µg

Cataloguenumber
PRO-928
Synonyms
Signalingthreshold-regulatingtransmembraneadapter1,SHP2-interactingtransmembraneadapterprotein,Suppression-inducingtransmembraneadapter1,gp30/40,SIT1,SIT,RP11-331F9.5,MGC125908,MGC125909,MGC125910.
Introduction
Signalingthreshold-regulatingtransmembraneadapter1(SIT1)negativelyregulatesT-cellantigenreceptor(TCR)-mediatedsignalinginT-cells.SIT1isinvolvedinpositiveselectionofT-cells.SIT1isspecificallyexpressedinT-andB-cells.SIT1isalsopresentinplasmacellsbutnotingerminalcenterB-cells(atproteinlevel).SIT1isexpressedinT-andB-celllymphoma.
Description
SIT1HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining156aminoacids(62-196a.a.)andhavingamolecularmassof16.9kDa(MolecularweightonSDS-PAGEwillappearhigher).SIT1isfusedtoa21aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
SIT1proteinsolution(0.25mg/ml)containing20mMTris-HClbuffer(pH8.0)and20%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMHLSQWTRGRSRSHPGQGRSGESVEEVPLYGNLHYLQTGRLSQDPEPDQQDPTLGGPARAAEEVMCYTSLQLRPPQGRIPGPGTPVKYSEVVLDSEPKSQASGPEPELYASVCAQTRRARASFPDQAYANSQPAAS.
SafetyDataSheet
SDS