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Other_recombinant_protein >
Prospec/NEFL Human/Neurofilament Light Human Recombinant/2µg/PRO-2584-2µg

Cataloguenumber
PRO-2584
Synonyms
Neurofilamentlightpolypeptide,NF-L,NEFL,NF68,NFL,68kDaneurofilamentprotein.
Introduction
NEFLorNeurofilamentlightpolypeptideisaproteinthatisencodedthroughtheNEFLgene.NEFLiscorrelatedtoadiseasecalledCharcot–Marie–Tooth.Theprotein’slightsubunitisdeterminedbyimmunoassaysintheplasmaandcerebrospinalfluid,ifpresent,itcanindicateonaxonaldamageinneurologicaldiseases.Bydoingso,NEFLcanactasaMarkerforHuntington"sdisease,AmyotrophicLateralSclerosisandmultiplesclerosis.
Description
NEFLHumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechain(2-543a.a)containing551aminoacidsincludinga9a.aN-terminalHistag.Thetotalmolecularmassis62.5kDa(calculated).
Source
PhysicalAppearance
Formulation
NEFLfiltered(0.4µm)andlyophilizedfrom0.5mg/mlsolutionin85mM,15mMTrisandGlycine,pH8.5.
Solubility
Itisrecommendedtoadd200µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MKHHHHHHASSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.