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Prospec/HLA-DRB1 Human, Sf9/Major Histocompatibility Complex Class II DR Beta 1 Human Recombinant, Sf9/5µg/PRO-2553-5µg

Cataloguenumber
PRO-2553
Synonyms
DRB1,HLADRB1,HLA-DR1B,HLA-DRB1,MHCclassIIantigenDRB116,DR-16,DR16,HumanLeucocyteAntigenDRB1,MHCClassIIHLA-DR-BetaCellSurfaceGlycoprotein,MHCClassIIHLA-DRw10-Beta.
Introduction
MajorHistocompatibilityComplexClassIIDRBeta1alsoknownasHLA-DRB1iaamemberoftheHLAclassIIbetachainparalogs.MoleculeclassIIisaheterodimerconsistingofanalpha(DRA)andabetachain(DRB),bothanchoredinthemembrane.HLA-DRB1takesanessentialpartintheimmunesystembypresentingpeptidesderivedfromextracellularproteins.ClassIImoleculesareexpressedinantigenpresentingcells(APC:Blymphocytes,dendriticcells,macrophages).FurThermore,thebetachainisapproximately26-28kDa.Itisencodedby6exons.Whileexononeencodestheleaderpeptide;exons2and3encodethetwoextracellulardomains;exon4encodesthetransmembranedomain;andexon5encodesthecytoplasmictail.
Description
HLA-DRB1producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining207aminoacids(30-227a.a.)andhavingamolecularmassof24.0kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).HLA-DRB1isexpressedwitha9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
HLA-DRB1proteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and30%glycerol.
StABIlity
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPGDTRPRFLWQPKRECHFFNGTERVRFLDRYFYNQEESVRFDSDVGEFRAVTELGRPDAEYWNSQKDILEQARAAVDTYCRHNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFLNGQEEKAGMVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSKHHHHHH
SafetyDataSheet
SDS