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Prospec/HSP27 Mouse/Heat Shock Protein 27 Mouse Recombinant/2µg/HSP-065-2µg

Cataloguenumber
HSP-065
Synonyms
Introduction
HSP27orEstrogen-Regulated24Kproteinisapartofthemammaliansmallheatshockproteinfamily.Theproteinisexpressedconstitutivelyinmanytissuesanditsexpressionisincreasedtohighlevelsafterdifferenttypesofstressincludinghightemperatures,toxicmetals,drugsandoxidants.FurThermore,Hsp27isphosphorylatedinvivoonthreephosphorylationsites(Ser15,Ser78andSer82)byproteinkinasesincludingMAPKAPkinase2andthestress-activatedproteinkinaseSAPK2(p38).
Description
HSP27MouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining232aminoacids(1-209a.a)andhavingamolecularmassof25.4kDa.HSP27isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheHSP27proteinsolution(1mg/ml)contains20mMTris-HCl(pH8.0),10%glyceroland1mMDTT.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMTERRVPFSLLRSPSWEPFRDWYPAHSRLFDQAFGVPRLPDEWSQWFSAAGWPGYVRPLPAATAEGPAAVTLAAPAFSRALNRQLSSGVSEIRQTADRWRVSLDVNHFAPEELTVKTKEGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTLVSSSLSPEGTLTVEAPLPKAVTQSAEITIPVTFEARAQIGGPEAGKSEQSGAK.
SafetyDataSheet
SDS