>
产品中心 >
Other_recombinant_protein >
Prospec/EIF3I Human, Sf9/Eukaryotic Translation Initiation Factor 3I Human Recombinant, Sf9/10µg/PRO-2611-10µg
Cataloguenumber
PRO-2611
Synonyms
eIF3-beta,eIF3-p36,EIF3S2,PRO2242,TRIP-1,TRIP1,Eukaryotictranslationinitiationfactor3subunitI,eIF3i,TGF-betareceptor-interactingprotein1,eIF-3-beta.
Introduction
Eukaryotictranslationinitiationfactor3,subunitI(EIF3I)ispartoftheeukaryotictranslationinitiationfactor3(eIF-3)complex,whichisessentialfornumerousstepsintheinitiationofproteinsynthesis.TheeIF-3complexlinkswiththe40SribosomeandfacilitatestherecruitmentofeIF-1,eIF-1A,eIF-2:GTP:methionyl-tRNAiandeIF-5toformthe43Spre-initiationcomplex(43SPIC).TheeIF-3complexstimulatesmRNArecruitmenttothe43SPICandscanningofthemRNAforAUGrecognition.TheeIF-3complexisalsoessentialfordisassemblyandrecyclingofpost-terminationribosomalcomplexesandsubsequentlypreventsprematurejoiningofthe40Sand60Sribosomalsubunitspriortoinitiation.AmongthediseasesassociatedwithEIF3Iareclonorchiasis,andtonsillitis.
Description
EIF3IHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining331aminoacids(1-325a.a)andhavingamolecularmassof37.3kDa(Migratesat40-57kDaonSDS-PAGEunderreducingconditions).EIF3Iisfusedtoan6aminoacidHis-tagatC-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
EIF3Iproteinsolution(0.25mg/ml)20mMTris-HClbuffer(pH8.0),0.1MNaCl,40%Glyceroland1mMDTT.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MKPILLQGHERSITQIKYNREGDLLFTVAKDPIVNVWYSVNGERLGTYMGHTGAVWCVDADWDTKHVLTGSADNSCRLWDCETGKQLALLKTNSAVRTCGFDFGGNIIMFSTDKQMGYQCFVSFFDLRDPSQIDNNEPYMKIPCNDSKITSAVWGPLGECIIAGHESGELNQYSAKSGEVLVNVKEHSRQINDIQLSRDMTMFVTASKDNTAKLFDSTTLEHQKTFRTERPVNSAALSPNYDHVVLGGGQEAMDVTTTSTRIGKFEARFFHLAFEEEFGRVKGHFGPINSVAFHPDGKSYSSGGEDGYVRIHYFDPQYFEFEFEAHHHHHH.
SafetyDataSheet
SDS
