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Prospec/AGO2 Human/Argonaute 2 Human Recombinant/10µg/PRO-2577-10µg

Cataloguenumber
PRO-2577
Synonyms
Proteinargonaute-2,Argonaute2,hAgo2,ArgonauteRISCcatalyticcomponent2,Eukaryotictranslationinitiationfactor2C2,eIF-2C2,eIF2C2,PAZPiwidomainprotein,PPD,AGO2,EIF2C2,Proteinslicer.
Introduction
TheArgonauteproteinispartoftheRISCorRNA-inducedsilencingcomplex,asso,theproteinhasakeypartintheslicingprocessesofRNA.TheRNAinterference(RNAi)isbeingheldbyRISC.Smallnon-codingRNAfragmentsbondtotheArgonauteproteins,throughbasepairing,eventuallyleadstothecleavageofmessengerRNAortranslationsuppression.
Description
AGO2HumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechain(a.a1-859)containing869aminoacidsincludinga10a.aN-terminalHistag.Thetotalmolecularmassis98.4kDa(calculated).
Source
PhysicalAppearance
Formulation
AGO2isfiltered(0.4µm)andlyophilizedfrom0.5mg/mlsolutionin50mMacetatebuffer,pH4.
Solubility
Itisrecommendedtoadd0.1Macetatebuffer,pH4toprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.AGO2isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MKHHHHHHASMYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.