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Prospec/DCUN1D2 Human/DCN1 Defective in Cullin Neddylation 1 Domain Containing 2 Human Recombinant/5µg/PRO-899-5µg

Cataloguenumber
PRO-899
Synonyms
DCN1-likeprotein2,DCUN1domain-containingprotein2,Defectiveincullinneddylationprotein1-likeprotein2,DCUN1D2,C13orf17,DCUN1L2.
Introduction
DCN1-likeprotein2(DCUN1D2)whichcontainsoneDCUN1domainand1UBA-likedomain,mayhavearoleintheneddylationofcullinswhichregulateSCF-typeubiquitinligasecomplexes.ThegeneencodingDcun1D2islocatedonhumanchromosome13.Chromosome13containsmajortumorsuppressorgenes,includingBRCA2andRB1,whicharelinkedwithbreastcancersusceptibilityandretinoblastoma,respectively.
Description
DCUN1D2HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining279aminoacids(1-259a.a.)andhavingamolecularmassof32.3kDa.DCUN1D2isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
DCUN1D2proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,30%glyceroland0.1MNaCl.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMHKLKSSQKDKVRQFMACTQAGERTAIYCLTQNEWRLDEATDSFFQNPDSLHRESMRNAVDKKKLERLYGRYKDPQDENKIGVDGIQQFCDDLSLDPASISVLVIAWKFRAATQCEFSRKEFLDGMTELGCDSMEKLKALLPRLEQELKDTAKFKDFYQFTFTFAKNPGQKGLDLEMAVAYWKLVLSGRFKFLDLWNTFLMEHHKRSIPRDTWNLLLDFGNMIADDMSNYDEEGAWPVLIDDFVEYARPVVTGGKRSLF.
SafetyDataSheet
SDS