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Prospec/CYB5A Human, Sf9/Cytochrome B5 Type A, Sf9 Human Recombinant/2µg/PRO-2555-2µg

Cataloguenumber
PRO-2555
Synonyms
Cytochromeb5isoform1,CYB5A,CYB5,MCB5,Microsomalcytochromeb5typeA.
Introduction
Cytochromeb5isoform1orCYB5A,ispartofthecytochromeb5familywhichisboundtothecellmembrane.CYB5Ahasakeyroleinthestearyl-CoA-desaturaseactivityasitreducesferrichemoglobintoferroushemoglobin.Cytochromeb5isoform1performsasanelectroncarrierfornumerousoxygenasesthatareimbeddedinthecellmembrane.
Description
CYB5AproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining117aminoacids(1-108a.a.)andhavingamolecularmassof13.3kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately18-28kDa).CYB5Aisexpressedwitha9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
CYB5Aproteinsolution(1mg/ml)containsphosphatebufferedsaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPMAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSHHHHHH.
SafetyDataSheet
SDS