>
产品中心 >
Other_recombinant_protein >
Prospec/COL4A3 Human/Collagen Type IV Alpha 3 Human Recombinant/10µg/PRO-387-10µg

Cataloguenumber
PRO-387
Synonyms
Introduction
Description
Humanα3chainofcollagenIV;identicalwiththeantigencalled"glomerularbasalmembraneantigen"(GBM).Recombinantantigenforsolid(ELISA)andfluidphasediagnosticassays.CalculatedMolecularweight:53kDa.Calculatedisoelectricpoint:pH8.9.
CDNAcodingforaminicollagenversionofthehumancollagenIVα3chainfusedtoahexa-histidinepurificationtag.Thetermminicollagendesignatestheremovalofmostoftheepitope-lesstriplehelicalcollagenousregion(situatedbetweentheN-terminal7SdomainandtheC-terminalnoncollagenousNC1domain),whichisarequirementforrecombinantproductionofthisantigen.
Source
PhysicalAppearance
Formulation
Theproteinsolutioncontains20mMHepes,pH8.0and4MUrea.
StABIlity
Recommendationsforstoragebuffer:neutraltoslightlyalkalinepHand4Mureaasdissociatingagent.
Storagetemperature:-70°Cto-80°C.
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Coatingconcentration
0.12-0.5μg/ml(dependingonthetypeofELISAplateandcoatingbuffer).Suitableforlabelingoffunctionalgroups.
Note
IthasbeenreportedthattheimmunodominantepitopeofGBMisacrypticepitopethatisnoteasilyaccessIBLetothecorrespondingautoantibodies.Itisnecessarytotreattheproteinundernonreducingconditionswithadenaturantsuchasureatounmasktheepitopes(seeHellmarketal.inAutoantibodies,Peter,J.B.andShoenfeld,Y.,eds.,ElsevierB.V.,1996,pp291-298).
Immunologicalfunctions
1.BindsIgG-typehumanauto-antibodies.
2.StandardELISAtest(checkerboardanalysisofpositive/negativesamples.
3.Immunodottestwithpositive/negativesamples.
SafetyDataSheet
SDS