>
产品中心 >
Other_recombinant_protein >
Prospec/CDC34 Human/Cell Division Cycle 34 Human Recombinant/100µg/ENZ-1103-100µg
Cataloguenumber
ENZ-1103
Synonyms
UB2R1,CDC-34,Ubiquitin-conjugatingenzymeE2R1,Ubiquitin-proteinligaseR1,Ubiquitin-conjugatingenzymeE2-32kDacomplementing,E2-CDC34,CDC34,EC6.3.2.19,UBC3,UBE2R1.
Introduction
CDC34takespartinthecontrolofcellcycleandDNAreplication.Cdc34inassociationwithdifferentE3complexes,includingSCF,targetsmanydifferentsubstratesforubiquitinationanddegradationduringcelldivision,signaltransduction,anddevelopment.Cdc34substratesthathavebeencharacterizedincludeB-Myb,Wee1,MyoD,ATF5,p27Xic1,andp27Kip1.Additionally,substratessuchasp21Cip1,E2F,cyclinE,andcyclinDareputativesubstratesofCdc34byvirtueoftheirSCFrequirementforproteolysis.Cdc34isself-associatethroughadomainintheC-terminus,andisphosphorylatedandubiquitinylatedinvivo.CDC34isusefulforinvitroubiquitinylationreactions.
Description
CellDivisionCycle34HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining236aminoacidsandhavingamolecularmassof26.7kDa.CDC34ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
CDC34issuppliedasa0.2μmfilteredsolutionconteining50mMHEPES,pH7.0,10%Glycerol,125mMNaCl,,5%Trehaloseand1mMDTT.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES.
SafetyDataSheet
SDS
