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Prospec/CEA Human, His/Carcinoembryonic Antigen Human Recombinant, His Tag/10µg/PRO-2407-10µg

Cataloguenumber
PRO-2407
Synonyms
CarcinoembryonicAntigenRelatedCellAdhesionMolecule5,CarcinoembryonicAntigen-RelatedCellAdhesionMolecule5,MeconiumAntigen100,CEA,CarcinoembryonicAntigen,CD66eAntigen,CD66e,Carcinoembryonicantigen-relatedcelladhesionmolecule5,Carcinoembryonicantigen,CEA.
Introduction
Carcinoembryonicantigen(CEA)isaglycoproteinpresentinfetaldigestive-tracttissues;it’sinvolvedincelladhesion.TheproductionofCEAstopsbeforebirth.CEAiscalledtumorMarkersinceitselevatedlevelsarefoundintheserumfromindividualswithcolorectal,gastric,pancreatic,lungandbreastcarcinomasandinheavysmokers.TherearealsobenignconditionsthatelevateCEAlevelssuchassmoking,infection,inflammatoryboweldisease,pancreatitis,cirrhosisoftheliver,andsomebenigntumors(intheequivalentorganswhichhavecancerswithelevatedCEA).Typically,higherlevelsofCEAarefoundinmen,smokers,andolderindividuals.ThepresenceofCEAassistsinscreening,inevaluatingrecurrentordisseminateddisease,andindeterminingthesuccessofsurgicalremovalofmalignanttumors.CEAlevelscanbeusedasindicatorsoftreatmentsuccess.Thenormalvaluesrangefrom0.0to2.5ng/mlofserum(fromblood),innon-smokers,agreateramountthanthatmaybesuggestiveofcancer.Levelsabove20ng/mlbeforetreatmentareassociatedwithcancerwhichhasalreadymetastasized.BenignconditionsdonotusuallycauseaCEAincreaseover10ng/ml.ThehighlevelsofCEAshouldreturntonormalaftersuccessfultherapy,howeverifduringfollowupthere’sanelevationinCEAlevelsitindicatesarecurrenceoftumor.Carcinoembryonicantigenfamilybelongstotheimmunoglobulinsuperfamily;itconsistsof29genes,18ofwhicharenormallyexpressed.
Description
CEAHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining659aminoacids(35-685a.a.)andhavingamolecularmassof72.3kDa(MolecularsizeonSDS-PAGEwillappearatapproximately70-150kDa).CEAisexpressedwithan8aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
CEAproteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
KLTIESTPFNVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYTLHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWVNNQSLPVSPRLQLSNGNRTLTLFNVTRNDTASYKCETQNPVSARRSDSVILNVLYGPDAPTISPLNTSYRSGENLNLSCHAASNPPAQYSWFVNGTFQQSTQELFIPNITVNNSGSYTCQAHNSDTGLNRTTVTTITVYAEPPKPFITSNNSNPVEDEDAVALTCEPEIQNTTYLWWVNNQSLPVSPRLQLSNDNRTLTLLSVTRNDVGPYECGIQNKLSVDHSDPVILNVLYGPDDPTISPSYTYYRPGVNLSLSCHAASNPPAQYSWLIDGNIQQHTQELFISNITEKNSGLYTCQANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASGTSPGLSALEHHHHHH.
SafetyDataSheet
SDS