>
产品中心 >
Other_recombinant_protein >
Prospec/BGN Mouse/Biglycan Mouse Recombinant/10µg/PRO-2537-10µg
Cataloguenumber
PRO-2537
Synonyms
BGN,DSPG1,MRLS,PG-S1,PGI,SEMDX,SLRR1A,Biglycan,Bone/cartilageproteoglycanI,BiglycanProteoglycan,MRLS.
Introduction
Biglycan(BGN)isasmallcellularorpericellularmatrixproteoglycanwhichtakespartinassemblyofcollagenfibrilsandmuscleregeneration.BGNiscloselycorrelatedinstructuretotwoothersmallproteoglycans,decorinandfibromodulin.BGNinteractswithseveralproteinsinvolvedinmusculardystrophy,includingalpha-dystroglycan,alpha-andgamma-sarcoglycanandcollagenVI.BGNisalsocriticalfortheassemblyofthedystrophin-associatedproteincomplex.
Description
BGNproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining574aminoacids(38-369a.a.)andhavingamolecularmassof64.6kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).
BGNisexpressedwitha242hIgG-His-tagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
TheBGNsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPDEEASGSDTTSGVPDLDSVTPTFSAMCPFGCHCHLRVVQCSDLGLKTVPKEISPDTTLLDLQNNDISELRKDDFKGLQHLYALVLVNNKISKIHEKAFSPLRKLQKLYISKNHLVEIPPNLPSSLVELRIHDNRIRKVPKGVFSGLRNMNCIEMGGNPLENSGFEPGAFDGLKLNYLRISEAKLTGIPKDLPETLNELHLDHNKIQAIELEDLLRYSKLYRLGLGHNQIRMIENGSLSFLPTLRELHLDNNKLSRVPAGLPDLKLLQVVYLHSNNITKVGINDFCPMGFGVKRAYYNGISLFNNPVPYWEVQPATFRCVTDRLAIQFGNYKKLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
SafetyDataSheet
SDS
