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Other_recombinant_protein >
Prospec/BGN Human, Sf9/Biglycan Human Recombinant, Sf9/2µg/PRO-2536-2µg
Cataloguenumber
PRO-2536
Synonyms
BGN,DSPG1,MRLS,PG-S1,PGI,SEMDX,SLRR1A,Biglycan,Bone/cartilageproteoglycanI,BiglycanProteoglycan,MRLS.
Introduction
Biglycan(BGN)isasmallcellularorpericellularmatrixproteoglycanwhichtakespartinassemblyofcollagenfibrilsandmuscleregeneration.BGNiscloselycorrelatedinstructuretotwoothersmallproteoglycans,decorinandfibromodulin.BGNinteractswithseveralproteinsinvolvedinmusculardystrophy,includingalpha-dystroglycan,alpha-andgamma-sarcoglycanandcollagenVI.BGNisalsocriticalfortheassemblyofthedystrophin-associatedproteincomplex.
Description
BGNproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining340aminoacids(38-368a.a.)andhavingamolecularmassof38.3kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).
BGNisexpressedwitha9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
TheBGNsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
TheED50forthiseffectis≤20ug/ml.Thespecificactivityismeasuredbyinhibitingthecellgrowthusing3T3‑L1mouseembryonicfibroblastADIpose-likecells.
Aminoacidsequence
ADPDEEASGADTSGVLDPDSVTPTYSAMCPFGCHCHLRVVQCSDLGLKSVPKEISPDTTLLDLQNNDISELRKDDFKGLQHLYALVLVNNKISKIHEKAFSPLRKLQKLYISKNHLVEIPPNLPSSLVELRIHDNRIRKVPKGVFSGLRNMNCIEMGGNPLENSGFEPGAFDGLKLNYLRISEAKLTGIPKDLPETLNELHLDHNKIQAIELEDLLRYSKLYRLGLGHNQIRMIENGSLSFLPTLRELHLDNNKLARVPSGLPDLKLLQVVYLHSNNITKVGVNDFCPMGFGVKRAYYNGISLFNNPVPYWEVQPATFRCVTDRLAIQFGNYKKHHHHHH.
SafetyDataSheet
SDS
