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Other_recombinant_protein >
Prospec/BIRC7 Human/Baculoviral IAP Repeat-Containing 7 Human Recombinant/5µg/PRO-612-5µg
Cataloguenumber
PRO-612
Synonyms
Introduction
BIRC7isapartoftheinhibitorofapoptosisprotein(IAP)familyandcontainsasinglecopyofabaculovirusIAPrepeat(BIR)aswellasaRING-typezincfingerdomain.TheBIRdomainisnecessaryforinhibitoryactivity&cooperateswithcaspases,whiletheRINGfingerdomainsometimesincreasesanti-apoptoticactivitybutdoesn’tinhibitapoptosisbyitself.2transcriptvariantsencodingdiverseisoformshavebeenfoundforthisgene.The2isoformshavedissimilarantiapoptoticcharacteristics,withisoformaprotectingcellsfromapoptosisinducedbystaurosporineandisoformbdefendingcellsfromapoptosis.BIRC7hasdirectcontactwithseveralcaspasestogetherwithcaspase-3,caspase-7,&caspase–9.BIRC7inhibitstheactivationofcaspase-9inducedbyApaf-1,cytochromec,anddATP.
Description
BIRC7HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining318aminoacids(1-298a.a.)andhavingamolecularmassof34kDa.BIRC7isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
BIRC7proteinsolution(1mg/ml)containing20mMTrispH-8,1mMDTT,0.1MNaCl,2mMEDTAand20%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGPKDSAKCLHRGPQPSHWAAGDGPTQERCGPRSLGSPVLGLDTCRAWDHVDGQILGQLRPLTEEEEEEGAGATLSRGPAFPGMGSEELRLASFYDWPLTAEVPPELLAAAGFFHTGHQDKVRCFFCYGGLQSWKRGDDPWTEHAKWFPSCQFLLRSKGRDFVHSVQETHSQLLGSWDPWEEPEDAAPVAPSVPASGYPELPTPRREVQSESAQEPGGVSPAEAQRAWWVLEPPGARDVEAQLRRLQEERTCKVCLDRAVSIVFVPCGHLVCAECAPGLQLCPICRAPVRSRVRTFLS.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
SafetyDataSheet
SDS
