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Prospec/Norovirus Group-1/Norovirus Group-1 Capsid Recombinant/500µg/NRV-213-500µg
Cataloguenumber
NRV-213
Introduction
Humannorovirusisclassifiedintotwogroups,group1&group2.Norwalkvirusisthespecieswhichbelongstogroup1andwasdiscoveredin1968atOhio.Norovirusisafamiliarviruswhichcauseshumangastroenteritiswiththefollowingsymptomsvomiting,diarrheaandsickness.CDCreportrevealedthatthereare19-21millionAmericansinfectedbyNororvirusannuallywith800deaths,1in15peoplewithinfection.Aroundtheworld,thisvirusaffectsabout267millionpeopleandcausesover200,000deathseachyear;theselossesaremostlyinlessdevelopedcountriesandintheveryyoung,elderlyandimmuno-suppressedpopulation,though,mostcasesareself-limitedwithafullrecoverywithinjustafewdays.Norovirusisextremelycontagiousandcanspreadfromhumantohumanthroughinfectedfood,waterorcontaminatedsurfaces.TheoutbreaksusuallyoccurfromNovember-April,whilethepeakisinJanuary.NorovirusisapositivesenseRNAviruswith7.5kbnucleotides,encodingamajorstructuralproteinVP1with50-55kDa.ThefulllengthofVP1capsidcomprisestheinternalN-terminal,Hinge,shell(S)andprotruding(P)domains.Pdomainfrom225to520formsP1-P2-P1structure.Moreover,Pdomainhasareceptorbindingregionwhichrecognizeshumanhisto-bloodgroupantigens(HBGAs).PdomainexpressedinbacteriacanspontaneouslyformaPdimeaswellasaPparticleaggregatedby12Pdimmers.PparticledisplaysanincreasedbindingactivitytoHBGAshigherthanvirus-likeparticle(VLP)formedbythefull-lengthcapsid.Fornorovirusvaccinedevelopment,weconsiderPdomainasagoodcandidate.
Description
TheRecombinantNorovirusGroup-1Capsid,E.Coliderived,isapositivesenseRNAviruswith7.5kbnucleotides,encodingamajorstructuralproteinVP1with50-55kDaandaVP2protein.ThefulllengthofVP1capsidproteinisderivedfromthegroup1Norwalkvirus.Theproteinisfusedtoa6HistagatN-terminalandpurifiedbychromatographytechniques.
Source
PhysicalAppearance
Formulation
25mMTrisBaseand25mMk2co3.
Purity
Proteinis>90%pureasdeterminedby12%PAGE(coomassiestaining).
StABIlity
TheRecombinantNorovirusGroup-1althoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
SafetyDataSheet
SDS
