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Prospec/Dengue-HRP/Dengue Horseradish Peroxidase Recombinant/100µg/DEN-044-100µg

Cataloguenumber
DEN-044
Introduction
TheDENVissmallstructurethatcanonlyreproduceinsideahostorganism.Thereare4relateddengueviruses-Degue-1,Dengue-2,Dengue-3,&Dengue4.Allofthemarepresentinthesameareasoftheworld.Thedenguevirushasjustaboutasphereshapedstructurecomprisedoftheviralgenomeandcapsidproteinsborderedbyanenvelopeandashellofantigen.Subsequentlyinfecting,thedenguevirustakesoverthehostcell"ssystemtoreplicatetheviralRNAgenomeandviralantigens.Oncematured,theresynthesizedDENVsaredischargedandcontinuetoinfectadditionalhostcells.
Description
RecombinantDengue-HRPisgeneticallydesigneddengueproteinconjugatedtoHorserADIshperoxidasewhichdetectslowtiterdengueantibodieshavingasharpODvaluecomparedtothecontrolwithoptimaldilutions.TheproprietaryDengue-HRPproteinsequenceisdesignedforbroadreactionstospecificantibodiesproducedfromthedifferentSEROtypesofdengueinfection.PotentialapplicationsforDengue-HRPincludeindirectELISAforthedetectionofdengueIgGorMACELISAforthedetectionofdengueIgM.
Source
PhysicalAppearance
Formulation
Phosphatebufferedsaline,pH7.4and25%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Applications
IndirectELISAandMAC-ELISA1:100-200dilutedDengue-HRPproduced3to6foldODvaluecomparedtoODfromthecontrol.TheODvaluemayvarydependingontheconcentrationofspecificIgGorIgM,samplefolddilution,sampleloadingvolumeandothervariables.
AssayConditions
Coateachwellwith1to3ug/wellpolyclonalormonoclonalanti-humanIgGorIgM.Leavein4Cforovernight.Washwithwashbufferandblockwith3%goatserumPBSfor1hourat37C.Washwithwashbufferagain.1:20dilutionofsamplewithPBS.Add100uldilutedsampleintoeachwell.Leavein37oCfor1hour.Washwithwashbufferagain.1:100to200diluteDen-HRPwithPBSandadd100ulintoeachwell.Incubatefor1hourat37C.Afterwashwithwashbufferandadd100ulsubstrateforcolorreaction.
SafetyDataSheet
SDS