
Cataloguenumber
DEN-037
Introduction
Description
Source
Purity
Proteinis>95%pureasdeterminedby10%PAGE(coomassiestaining).
Formulation
Phosphatebufferedsaline,0.02%sodiumnitrateand25mMK2CO3.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
SafetyDataSheet
SDS
Preparationprotocol
Followingistheprotocolneededtodeveloptherapidtestbythisantigen:1.Prepare&lamBDa;522to525freshgoldparticlewithin1-2days,adjustODto1.2to1.4.2.conjugateatpH7.2-7.5ataconcentrationof16ug/ml;3.conjugatefor2-4hours,thenadd25mMphosphatebufferwith0.2%PVP10,000pH8.0tosUSPendtheconjugategoldpellet.4.Blockthesuspensionwith10%BSAtofinal1%BSA.5.Usethesamplepadtreatedwith50mMborate,1%Tween-20and0.5%PVP10,000,pH9.2.6.Preparethesamplebufferwith50-100mMcarbonatebufferwith0.5-1.0%Tween-20.Therapidtestpreparedbythisantigenhasover90%sensitivityandspecificityover90%todetectdengueIgMandIgG.