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Prospec/CMV Mosaic/Cytomegalo Virus Mosaic Recombinant/500µg/CMV-217-500µg
Cataloguenumber
CMV-217
Introduction
CMVbelongstotheBetaherpesvirinaesubfamilyofHerpesviridaewhichincludesherpessimplexvirustypes1and2,varicella-zostervirus,andEpstein-Barrvirus.TheherpesvirusesshareacharacteristicABIlitytoremainlatentoverlongperiods.CMVisadouble-strandedlinearDNAviruswith162hexagonalproteincapsomeressurroundedbyalipidmembrane.CMVhasthelargestgenomeoftheherpesviruses,rangingfrom230-240kilobasepairs.HumanCMViscomposedofuniqueandinvertedrepeatsthatincludetheexistenceof4genomeisomerscausedbyinversionofL-Sgenomecomponents(classE).Replicationmaybedividedintoimmediateearly,delayedearly,andlategeneexpressionbasedontimeofsynthesisafterinfection.TheDNAisreplicatedbyrollingcircles.Invitro,CMVreplicatesinhumanfibroblasts.
Description
TheE.ColiderivedRecombinantCytomegaloVirusMosaiccontainsthemultipleepitopesfromP150-P52-P38-P65-P28havingamolecularweightof40kDa.TheCMVMosaicisfusedtoa6xHistagandpurifiedbyproprietarychromatographictechnique.
Source
PhysicalAppearance
Formulation
CMVMosaicproteinsolution(0.42mg/ml)containsPhosphatebufferedsaline,2Mureaand100mMarginine.
Stability
CMVMosaicproteinalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.Pleasepreventfreezethawcycles.
Purity
CMVMosaicis>95%pureasdeterminedby12%PAGE(coomassiestaining).
SafetyDataSheet
SDS