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Prospec/TPSAB1 Human, Sf9/Tryptase Alpha/Beta 1 Human Recombinant, Sf9/10µg/ENZ-1062-10µg
Cataloguenumber
ENZ-1062
Synonyms
Tryptasealpha/beta-1,TPSAB1,TPS1,TPS2,TPSB1,TryptaseI,Tryptasealpha-1.
Introduction
Tryptasealpha/beta-1(TPSAB1)isatryptasewhichisthekeyneutralproteasepresentinmastcellsandisdischargeduponthecoupledactivation-degranulationresponseofthiscelltype.TPSAB1isenzymaticallyactiveonlyasaheparin-stABIlizedtetramer,andisresistanttoallknownendogenousproteinaseinhibitors.TPSAB1isimplicatedasamediatorinthepathogenesisofasthmaandotherallergicandinflammatorydisorders.
Description
TPSAB1producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechain(31-275a.a.)andfusedtoa6aaHisTagatC-terminuscontainingatotalof251aminoacidsandhavingamolecularmassof28.2kDa.TPSAB1showsmultiplebandsbetween28-40kDaonSDS-PAGE,reducingconditionsandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TPSAB1proteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)&20%glycerol.
Stability
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
IVGGQEAPRSKWPWQVSLRVHGPYWMHFCGGSLIHPQWVLTAAHCVGPDVKDLAALRVQLREQHLYYQDQLLPVSRIIVHPQFYTAQIGADIALLELEEPVNVSSHVHTVTLPPASETFPPGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKKPHHHHHH.
SafetyDataSheet
SDS

