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Prospec/GALNT1 Mouse/Polypeptide N-Acetylgalactosaminyltransferase 1 Mouse Recombinant/1µg/ENZ-1066-1µg
Cataloguenumber
ENZ-1066
Synonyms
PolypeptideN-acetylgalactosaminyltransferase1,PolypeptideGalNActransferase1,GalNAc-T1,pp-GaNTase1,Protein-UDPacetylgalactosaminyltransferase1,polypeptideN-acetylgalactosaminyltransferase1.
Introduction
PolypeptideN-Acetylgalactosaminyltransferase1(Galnt1),ispartoftheUDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase(GalNAc-T)familyofenzymes.TheinitialreactioninO-linkedoligosaccharidebiosynthesisiscatalyzedbyGlant1,thetransferofanN-acetyl-D-galactosamineresiduetoaserineorthreonineresidueontheproteinreceptor.Moreover,Galnt1isimplicatedintheglycosylationofproteinsvitalforboneformationforinstanceosteopontinandbonesialoprotein.
Description
GALNT1producedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining528aminoacids(41-559a.a.)andhavingamolecularmassof60.5kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).GALNT1isexpressedwithan9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
GALNT1proteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPGLPAGDVLELVQKPHEGPGEMGKPVVIPKEDQEKMKEMFKINQFNLMASEMIALNRSLPDVRLEGCKTKVYPDNLPTTSVVIVFHNEAWSTLLRTVHSVINRSPRHMIEEIVLVDDASERDFLKRPLESYVKKLKVPVHVIRMEQRSGLIRARLKGAAVSRGQVITFLDAHCECTAGWLEPLLARIKHDRRTVVCPIIDVISDDTFEYMAGSDMTYGGFNWKLNFRWYPVPQREMDRRKGDRTLPVRTPTMAGGLFSIDRDYFQEIGTYDAGMDIWGGENLEISFRIWQCGGTLEIVTCSHVGHVFRKATPYTFPGGTGQIINKNNRRLAEVWMDEFKNFFYIISPGVTKVDYGDISSRLGLRRKLQCKPFSWYLENIYPDSQIPRHYFSLGEIRNVETNQCLDNMARKENEKVGIFNCHGMGGNQVFSYTANKEIRTDDLCLDVSKLNGPVTMLKCHHLKGNQLWEYDPVKLTLQHVNSNQCLDKATEEDSQVPSIRDCTGSRSQQWLLRNVTLPEIFHHHHHH.
SafetyDataSheet
SDS
