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Prospec/GOT2 Mouse, Active/Glutamic-Oxaloacetic Transaminase 2, Active Mouse Recombinant/2µg/ENZ-1111-2µg
Cataloguenumber
ENZ-1111
Synonyms
TransaminaseA,KAT4,KATIV,KAT-4,KAT-IV,KynurenineAminotransferase4.
Introduction
GOT2isapyridoxalphosphate-dependentenzymewhichisfoundincytoplasmicandinner-membranemitochondrialforms,GOT1andGOT2.GOT2participatesinaminoacidmetabolismandtheureaandtricarboxylicacidcycles.Bothenzymesarehomodimericanddemonstrateclosehomology.
Description
GOT2MouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining422aminoacids(30-430aa)andhavingamolecularmassof46.8kDa.GOT2isfusedtoa21aminoacidHistagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
TheGOT2solution(0.5mg/ml)contains10%GlycerolandPhosphateBufferedSaline(pH7.4)
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Specificactivityis>20units/mg.Measuredbytheamountofenzymethatconverts1umoleofalpha-ketoglutaratetoL-GlutamateperminuteatpH8.0at25C˚.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFCKASAELALGENNEVLKSGRFVTVQTISGTGALRVGASFLQRFFKFSRDVFLPKPSWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFSGALEDISKIPEQSVLLLHACAHNPTGVDPRPEQWKEIASVVKKKNLFAFFDMAYQGFASGDGDKDAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTVVCKDAEEAKRVESQLKILIRPLYSNPPLNGARIAATILTSPDLRKQWLQEVKGMADRIISMRTQLVSNLKKEGSSHNWQHITDQIGMFCFTGLKPEQVERLTKEFSVYMTKDGRISVAGVTSGNVGYLAHAIHQVTK
SafetyDataSheet
SDS

