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Prospec/GOT2 Mouse/Glutamic-Oxaloacetic Transaminase 2 Mouse Recombinant/10µg/ENZ-1095-10µg
Cataloguenumber
ENZ-1095
Synonyms
TransaminaseA,KAT4,KATIV,KAT-4,KAT-IV,KynurenineAminotransferase4.
Introduction
GOT2isapyridoxalphosphate-dependentenzymewhichisfoundincytoplasmicandinner-membranemitochondrialforms,GOT1andGOT2.GOT2takespartinaminoacidmetabolismandtheureaandtricarboxylicacidcycles.Bothenzymesarehomodimericanddemonstrateclosehomology.
Description
GOT2RecombinantMouseproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining424aminoacids(30-430a.a.)andhavingamolecularmassof46.8kDa.MouseGOT2isfusedtoa21aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheGOT2solution(0.5mg/ml)containsPBS,pH7.4and10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Activityisgreaterthan20units/mg,andisdefinedastheamountofenzymethatconverts1umoleofalpha-ketoglutaratetoL-GlutamateperminuteatpH8.0at25℃.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFCKASAELALGENNEVLKSGRFVTVQTISGTGALRVGASFLQRFFKFSRDVFLPKPSWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFSGALEDISKIPEQSVLLLHACAHNPTGVDPRPEQWKEIASVVKKKNLFAFFDMAYQGFASGDGDKDAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTVVCKDAEEAKRVESQLKILIRPLYSNPPLNGARIAATILTSPDLRKQWLQEVKGMADRIISMRTQLVSNLKKEGSSHNWQHITDQIGMFCFTGLKPEQVERLTKEFSVYMTKDGRISVAGVTSGNVGYLAHAIHQV.
SafetyDataSheet
SDS

