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Prospec/UMPS Human, Sf9/Uridine Monophosphate Synthetase Human Recombinant, Sf9/2µg/ENZ-1057-2µg
Cataloguenumber
ENZ-1057
Synonyms
UridineMonophosphateSynthetase,UMPSynthase,OrotatePhosphoribosylTransferaseAndOrotidine-5-Decarboxylase,Orotidine5-PhosphateDecarboxylase,OrotatePhosphoribosyltransferase,Uridine5-MonophosphateSynthase,OMPdecase,OPRTase,OPRT,Uridine5"-monophosphatesynthase,UMPsynthase,Orotatephosphoribosyltransferase,Orotidine5"-phosphatedecarboxylase,ODC.
Introduction
Uridine5"-monophosphatesynthase(UMPS),isabifunctionalenzymethatcatalyzestheultimatetwostepsofthedenovopyrimidinebiosyntheticpathway.UMPSineukaryoteslinkstheorotatephosphoribosyltransferaseandtheorotidine-5’-monophosphate(OMP)decarboxylaseactivitiesintoasingleprotein.Theharmonyofthese2enzymesisassumedtobestABIlizedthecatalyticcentersasaresultofthelowmolarconcentrationoftheproteininmammaliancells.MutationsinUMPSarethereasonofinheritedoroticaciduriadisease.
Description
UMPSHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining486aminoacids(1-480a.a.)andhavingamolecularmassof53kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).UMPSisexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
UMPSproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4).
Stability
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIVSRPRLLSQVADILFQTAQNAGISFDTVCGVPYTALPLATVICSTNQIPMLIRRKETKDYGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQKEGLKVTDAIVLLDREQGGKDKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRFIQENVFVAANHNGSPLSIKEAPKELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPSICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIASWADLVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEEHSEFVVGFISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVGRGIISAADRLEAAEMYRKAAWEAYLSRLGVHHHHHH.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

