Cataloguenumber
ENZ-1081
Synonyms
AA986712,D5Ertd135e,SecS,SLA,SLA/LPautoantigen,SLA-p35,Selenocysteinyl-tRNA(Sec)synthase,Selenocysteinesynthase,Liver-pancreasantigen,Solubleliverantigen,Secsynthase,UGAsuppressortRNA-associatedprotein,SepSecS.
Introduction
SEPSECScatalyzesthelaststepofsecsynthesisbyconvertingO-phosphoseryl-tRNA(sec)toselenocysteinyl-tRNA(sec)usingselenophosphateastheseleniumdonor.FurThermore,SEPSECSproteinisconsideredaspecificMarkerofautoimmunehepatitis.
Description
SEPSECSproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining527aminoacids(1-504a.a.)andhavingamolecularmassof57.7kDa.SEPSECSisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheSEPSECSproteinsolution(0.25mg/ml)isformulatedin20mMTris-HClbuffer(pH7.5)1mMDTT,0.2MNaCland50%glycerol.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMNPESFAAGERRVSPAYVRQGCEARRAHEHLIRLLLEQGKCPEDGWDESTLELFLHELAVMDSNNFLGNCGVGEREGRVASALVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLNVIKLAGVHSVASCFVVPMATGMSLTLCFLTLRH
KRPKAKYIIWPRIDQKSCFKSMVTAGFEPVVIENVLEGDELRTDLKAVEAKIQELGPEHILCLHSTTACFAPRVPDRLEELAVICANYDIPHVVNNAYGLQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNEPFIQDISKMYPGRASASPSLDVLITLLSLGCSGYRKL
LKERKEMFVYLSTQLKKLAEAHNERLLQTPHNPISLAMTLKTIDGHHDKAVTQLGSMLFTRQVSGARAVPLGNVQTVSGHTFRGFMSHADNYPCAYLNAAAAIGMKMQDVDLFIKRLDKCLNIVRKEQTRASVVSGADRNKAEDADIEEMALKLDDVLGDVGQGPAL.
Purity
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles
SafetyDataSheet
SDS

