Cataloguenumber
PKA-024
Synonyms
Lymphokine-activatedkillerT-cell-originatedproteinkinase,Cancer/testisantigen84,CT84,MAPKK-likeproteinkinase,Nori-3,PDZ-bindingkinase,Spermatogenesis-relatedproteinkinase,SPK,T-LAKcell-originatedproteinkinase,PBK,TOPK.
Introduction
Lymphokine-activatedkillerT-cell-originatedproteinkinase(PBK)isaserine/threoninekinaserelatedtothedualspecificmitogen-activatedproteinkinase(MAPKK)family.PBKisabundantinplacenta(isalsofoundinthetestis’outercelllayerofseminiferoustubules)andabsentfromadultbraintissue.PBKisinvolvedintheactivationoflymphoidcellsandsupportstesticularfunctions,withapossIBLeroleintheprocessofspermatogenesis.MitoticphosphorylationisrequiredforPBK’scatalyticactivity.Oncephosphorylated,PBKformsacomplexwithTP53,leADIngtoTP53destABIlizationandweakeningofG2/McheckpointduringsomekindofDNAdamage.PBKisactiveonlyduringmitosis.Inaddition,aPDZdomaininthetumorsuppressorproteinDlgcancoordinatewiththeT/SXVmotifofPBK.PBKalsophosphorylatesMAPkinasep38,andmayhavearoleintheactivationoflymphoidcells.
Description
PBKHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining346aminoacids(1-322a.a)andhavingamolecularmassof38.6kDa(MolecularweightonSDS-PAGEwillappearhigher).PBKisfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
DCKissuppliedasasterilefilteredclearsolution.
Formulation
PBKproteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,10%glyceroland0.1MNaCl.
Stability
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPAAIILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALETDV. SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.