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Prospec/PTPN1 Human/Protein Tyrosine Phosphatase Non Receptor Type-1 Human Recombinant/10µg/PKA-219-10µg
Cataloguenumber
PKA-219
Synonyms
Introduction
ProteinTyrosinePhosphatase1Bisthefoundingmemberoftheproteintyrosinephosphatase(PTP)family,whichwasisolatedandidentifiedbasedonitsenzymaticactivityandaminoacidsequence.PTPscatalyzethehydrolysisofthephosphatemonoestersspecificallyontyrosineresidues.MembersofthePTPfamilyshareahighlyconservedcatalyticmotif,whichisessentialforthecatalyticactivity.PTPsareknowntobesignalingmoleculesthatregulateavarietyofcellularprocessesincludingcellgrowth,differentiation,mitoticcycle,andoncogenictransformation.ThisPTPhasbeenshowntoactasanegativeregulatorofINSsignalingbydephosphorylatingthephosphotryosineresiduesofINSreceptorkinase.ThisPTPwasalsoreportedtodephosphorylateepidermalgrowthfactorreceptorkinase,aswellasJAK2andTYK2kinases,whichimplicatedtheroleofthisPTPincellgrowthcontrol,andcellresponsetoIFNstimulation.
Description
Source
PhysicalAppearance
Formulation
StABIlity
Purity
Aminoacidsequence
BIOLOGicalActivity
AssayConditions
1)Prepare180µlassaybufferintoasuitablecontainer:Thefinalconcentrationsare11mMTris-HCl(pH7.5),55mMNaCl,2.2mMDTT,1.1mMMnCl2,1.1mMEDTAand33mMpNPP.2)Equilibrateto37°CandmonitoratA405nmuntilthevalueisconstantusingaspectrophotometer.3)Add20µlofrecombinantPTP-1Bprotein12.5ug/mlin0.1%BSAinDW.4)RecordtheincreaseinA405nmfor5minutes.
SafetyDataSheet
SDS

