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Prospec/Benzonase Nuclease /Benzonase Nuclease Serratia Marcescens Recombinant/5000U/ENZ-1112-5000U
Cataloguenumber
ENZ-1112
Introduction
Serratiamarcescenssecretesanendonucleasethathasexceptionallyhighspecificactivitytothemediumthatsurroundsit.TheBenzonaseNucleaseismainlyusedforeliminationofnucleicacidcontaminationfrompurifiedproteins,downstreamprocessing,reductionofviscosityetc.Nucleicacidcontaminantsarecausedbynucleasereleasedtothemedium.TheDNAisbeingdestroyedbythereleaseoftheS.marcescensnucleaseanditactsasthekillergenefortheautodestructionofmicroorganisms.
Description
BenzonaseNucleaseSerratiaMarcescensRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining245aminoacidsandhavingamolecularmassof30kDawith2essentialdisulfidebonds.
BenzonaseNucleaseispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
TheBenzonaseNucleasesolutioncontains50%glycerol,50mMTris-HClpH8.0,20mMNaCland2mMMgCl2.
StABIlity
Purity
Greaterthan99.0%asdeterminedbySDS-PAGE.
UnitDefinition
1UBenzonaseNucleaseisdefinedastheamountofenzymethatcausesaΔA260of1in30min,whichcorrespondstocompletedigestionof37μgDNA.Standardreactionconditionsare1mg/mlsonicatedDNAsubstratein50mMTris-HClpH8.0,0.1mg/mlBSA,1mMMgCl2,incubatedat37°C.
Specificity
Unspecific(DNA,RNA)attacksallnucleicacids(singlestrand,doublestrand,circular,supercoiled)withnoapparentsequencepreference.Finalreactionproduct:
5’-mono-phosphateterminatedoligonucleotides(3-5bases).
ProteaseActivity:Notdetectable
SafetyDataSheet
SDS

