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Prospec/PPID Mouse/Peptidylprolyl Isomerase D Mouse Recombinant/5µg/ENZ-1069-5µg
Cataloguenumber
ENZ-1069
Synonyms
Peptidyl-prolylcis-transisomeraseD,PPIaseD,40kDapeptidyl-prolylcis-transisomerase,Cyclophilin-40,CYP-40,Cyclophilin-relatedprotein,CYP40,CYPD,PPID,PeptidylprolylIsomeraseD.
Introduction
Cyclophilin-Disamemberofthepeptidyl-prolylcis-transisomerase(PPIase)family.PPIasescatalyzethecis-transisomerizationofprolineimidicpeptidebondsinoligopeptidesandspeedsuptheproteinfolding.Cyclophilin-DpossessPPIaseactivityandbindstotheimmunosuppressantcyclosporin-A.Cyclophilin-Disverywellknownthatitsoverexpressionsuppressestheapoptosisincancercell.Cyclophilin-Dsuppressesapoptoticcelldeathbytheuseofmitochondrialhexokinase-2dependentmechanismincancercells.
Description
PPIDMouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining395aminoacids(1-370a.a.)andhavingamolecularmassof43.4kDa.PPIDisfusedtoa25aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
PPIDproteinsolution(1mg/ml)containing20mMTris-Hclbuffer(pH8.0),10%glyceroland1mMDTT.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSEFMSHASPAAKPSNSKNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGTGSTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGPNTNGSQFFITTVPTPHLDGKHVVFGQVIKGLGVARTLENVEVNGEKPAKLCVIAECGELKEGDDWGIFPKDGSGDSHPDFPEDADIDLKDVDKILLISEDLKNIGNTFFKSQNWEMAIKKYAKVLRYVDSSKAVIEKADRSRLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALEMDPSNTKALYRKAQGWQGLKEYDQALADLKKAQEIAPGDKAIQAELLKVKQMIKAQKDKEKAVYAKMFA.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Specificactivityis>700nmol/min/mg,andisdefinedastheamountofenzymethatcleaves1umoleofsuc-AAFP-PNAperminuteat37°CinTris–HClpH8.0usingchymotrypsin.
SafetyDataSheet
SDS
