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Prospec/GZMB Human, sf9/Granzyme-B, Sf9 Human Recombinant/1µg/ENZ-1078-1µg
Cataloguenumber
ENZ-1078
Synonyms
GranzymeB,T-CellSerineProtease1-3E,CathepsinG-Like1,GranzymeB(Granzyme2,CytotoxicT-Lymphocyte-AssociatedSerineEsterase1,CytotoxicT-LymphocyteProteinase2,CytotoxicSerineProteaseB,HumanLymphocyteProtein,Fragmentin2,EC3.4.21.79,CTSGL1,CTLA1,CSPB,CGL1,SECT,C11,HLP,CytotoxicT-Lymphocyte-AssociatedSerineEsterase1,LymphocyteProtease,Fragmentin-2,Granzyme2,Granzyme-2,EC3.4.21,CTLA-1,CGL-1,CSP-B,CCPI,GRB.
Introduction
Granzyme-B(GZMB)isessentialfortargetcelllysisincell-mediatedimmuneresponses.GZMBisrelatedtoanactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.GZMBcutscaspase-3,-7,-9and10toactivateenzymesmediatingapoptosis.
Description
GZMBproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining235aminoacids(19-247a.a.)andhavingamolecularmassof26.5kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).GZMBisexpressedwithan6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
GZMBproteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4),20%glyceroland1mMDTT.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Specificactivityis>7,000pmol/min/ug,andisdefinedastheamountofenzymethatcleave1pmoleofBoc-Ala-Ala-Asp-SBzlat37C.
Aminoacidsequence
GEIIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIRDDFVLTAAHCWGSSINVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRYHHHHHH
SafetyDataSheet
SDS

