>
产品中心 >
Other_enzymes >
Prospec/MDH2 Mouse/Malate Dehydrogenase 2 Mouse Recombinant/10µg/ENZ-1068-10µg
Cataloguenumber
ENZ-1068
Synonyms
Introduction
MDH2catalyzesthereversIBLeoxidationofmalatetooxaloacetate,utilizingtheNAD/NADHcofactorsysteminthecitricacidcycle.MDH2islocalizedtothemitochondriaandtakespartinthemalate-aspartateshuttlethatfunctionsinthemetaboliccoordinationbetweencytosolandmitochondria.MDH2ishighyexpressedintheadrenalsystem,smallintestine,heartandpancreas.
Description
MDH2MouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining335aminoacids(25-338a.a.)andhavingamolecularmassof35.4kDa.MDH2isfusedtoa21aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
MDH2proteinsolution(1mg/ml)containingPhosphatebufferedsaline(pH7.4)and20%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETRANVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMVCIIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLATLTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVQSKETECTYFSTPLLLGKKGLEKNLGIGKITPFEEKMIAEAIPELKASIKKGEDFVKNMK.
Purity
BIOLOGicalActivity
Specificactivityis>800units/mg,andisdefinedastheamountofenzymethatcleaves1umoleofoxaloacetateandbeta-NADHtoLmalateandbeta-NADperminuteatpH8.0at37°C.
SafetyDataSheet
SDS

