Cataloguenumber
PRO-2620
Synonyms
B7-2/CD86,CD86,B7-2,B7.2,B70,CD28LG2,LAB72,T-lymphocyteactivationantigenCD86,ActivationB7-2antigen,B70,BU63,CTLA-4counter-receptorB7.2,FUN-1.
Introduction
CD86,alsoknownasB7-2orClusterofDifferentiation86isaproteinnecessaryforTcellactivationandsurvival,itisexpressedoncellsthatareantigen-presentingcellsandactsascostimulatorysignalsforTcells.CD86istheligandforCD28(forautoregulationandintercellularassociation)andCTLA-4(forattenuationofregulationandcellulardisassociation),twodifferentproteins.
Description
CD86HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining223aminoacids(31-247a.a.)andhavingamolecularmassof25.6kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).CD86isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
CD86proteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
YFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIPHHHHHH.
SafetyDataSheet
SDS