Cataloguenumber
PRO-2516
Synonyms
Basiginisoform2,5F7,CD147,EMMPRIN,OK,TCSF,LeukocyteactivationantigenM6,OKbloodgroupantigen.
Introduction
BasiginIsoform2orBSGisapartoftheimmunoglobulinsuperfamily,itisatype1transmembraneproteinwhichhasavarietyofNandO-glycosylation.BSGhasseveralligands,suchasintegrins,cyclophilinproteinsCyp-A&CyP-B.Theproteinhasacrucialroleinintercellularrecognition,ittakespartinmanyimmunologicprocesses,differentiationandcelldevelopment.Also,itregulatesnumeruscellprocessessuchasproductionandreleaseofexpressionofthemono-carboxylatetransporter,spermatogenesis,etc.BasiginIsoform2promotesinvasion,metastasis,growthandsurvivalofmalignantcellsbyproducingandreleasingmatrixmetalloproteinasesnearbymesenchymalcellsandtumorcells.
Description
BSGproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining428aminoacids(22-207a.a.)andhavingamolecularmassof47.5kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).BSGisexpressedwitha242aminoacidhIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
BSGproteinsolution(0.5mg/ml)containsphosphatebufferedsaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPAAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLALEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
SafetyDataSheet
SDS