Cataloguenumber
PRO-2539
Synonyms
CD3dMolecule,CD3dAntigen,DeltaPolypeptide(TiT3Complex),CD3dMolecule,Delta(CD3-TCRComplex),T-CellReceptorT3DeltaChain,T3D,T-CellSurfaceGlycoproteinCD3DeltaChain,CD3Antigen,DeltaSubunit,OKT3,DeltaChain,CD3dAntigen,CD3Delta,CD3-DELTA,IMD19,CD3D.
Introduction
CD3D,alsoknownasDeltaPolypeptide(TiT3Complex),isasingle-passtype1membraneprotein.CD3DjointlywithCD3-zeta,CD3-gamma,CD3-epsilonandtheT-cellreceptoralpha/betaandgamma/deltaheterodimers,createstheT-cellreceptor-CD3complex.Onceantigenpresentingcells(APCs)activateT-cellreceptor(TCR),TCR-mediatedsignalsaretransferredthroughthecellmembranebytheCD3chainsCD3D,CD3E,CD3GandCD3Z.Likewise,CD3Dtakesanimportantpartinadaptiveimmuneresponseandparticipatesinthymocytedifferentiation.
Description
CD3DproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechain(22-105a.a.)andfusedtoa239aahIgG-His-tagatC-terminuscontainingatotalof323aminoacidsandhavingamolecularmassof36.5kDa.CD3Dshowsmultiplebandsbetween40-57kDaonSDS-PAGE,reducingconditionsandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
CD3Dproteinsolution(0.5mg/ml)contains10%glycerol&Phosphatebufferedsaline(pH7.4).
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
FKIPIEELEDRVFVNCNTSITWVEGTVGTLLSDITRLDLGKRILDPRGIYRCNGTDIYKDKESTVQVHYRMCQSCVELDPATVALEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
