Cataloguenumber
PRO-2492
Synonyms
MyeloidcellsurfaceantigenCD33isoform1,CD33,FLJ00391,p67,SIGLEC-3,SIGLEC3,gp67.
Introduction
CD33isputativeadhesionmoleculeofmyelomonocytic-derivedcellswhichmediatessialic-aciddependentbindingtocells.CD33preferstobindtoalpha-2,6-linkedsialicacid.Thesialicacidrecognitionsiteismaskedbycisinteractionswithsialicacidsonthesamecellsurface.Intheimmuneresponse,CD33performasaninhibitoryreceptoruponligandinducedtyrosinephosphorylationbyrecruitingcytoplasmicphosphatasesthroughtheirSH2domainswhichblocksignaltransductionthroughdephosphorylationofsignalingmolecules.Inaddition,CD33inducesapoptosisinacutemyeloidleukemia.
Description
CD33producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechain(18-259a.a.)andfusedtoa239aahIgG-HisTagatC-terminuscontainingatotalof484aminoacidsandhavingamolecularmassof54kDa.CD33showsmultiplebandsbetween50-70kDaonSDS-PAGE,reducingconditionsandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
CD33proteinsolution(0.25mg/ml)containsPhosphatebufferedsaline(pH7.4)&10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADLDPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVHLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
SafetyDataSheet
SDS