Cataloguenumber
PRO-2491
Synonyms
Antigen-presentingglycoproteinCD1d,R3G1,CDantigen:CD1d,DifferentiationAntigenCD1-Alpha-3,T-CellSurfaceGlycoproteinCD1d,ThymocyteAntigenCD1D,CD1A,R3,CD1dMolecule,CD1DAntigen,DPolypeptide,CD1dAntigen,R3G1,HMCClassI,Antigen-LikeGlycoproteinCD1D,Antigen-PresentingGlycoproteinCD1d.
Introduction
CD1D,alsoknownasantigen-presentingglycoproteinCD1d,isatransmembraneglycoproteinwhichbelongstotheCD1familyofglycolipidantigen-presentingMHC-likemolecules.CD1d-presentedlipidantigensactivateaspecialclassofTcells,familiarasnaturalkillerT(NKT)cells,duringtheinteractionwiththeT-cellreceptorpresentonNKTmembranes.Onceactivated,NKTcellsrapidlyproduceTh1&Th2cytokines,usuallyrepresentedbyinterleukin4production.
Description
CD1DproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining290aminoacids(20-301a.a.)andhavingamolecularmassof32.9kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).
CD1Disexpressedwithan8aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
CD1Dproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorage,itisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
EVPQRLFPLRCLQISSFANSSWTRTDGLAWLGELQTHSWSNDSDTVRSLKPWSQGTFSDQQWETLQHIFRVYRSSFTRDVKEFAKMLRLSYPLELQVSAGCEVHPGNASNNFFHVAFQGKDILSFQGTSWEPTQEAPLWVNLAIQVLNQDKWTRETVQWLLNGTCPQFVSGLLESGKSELKKQVKPKAWLSRGPSPGPGRLLLVCHVSGFYPKPVWVKWMRGEQEQQGTQPGDILPNADETWYLRATLDVVAGEAAGLSCRVKHSSLEGQDIVLYWGGSYTSLEHHHHHH.
SafetyDataSheet
SDS
