>
产品中心 >
Growth_factor >
Prospec/PF4 Bovine/Platelet Factor-4 (CXCL4) Bovine Recombinant/50µg/CHM-039-50µg
Cataloguenumber
CHM-039
Synonyms
CXCL4,PF-4,PF4,Iroplact,Oncostatin-A,SCYB4,MGC138298.
Introduction
Plateletfactor-4isa70-aminoacidproteinthatisreleasedfromthealpha-granulesofactivatedplatelets.PF4’smajorphysiologicroleappearstobeneutralizationofmoleculesontheendothelialsurfaceofbloodvessels,therebyinhibitinglocalantithrombinIIIactivityandpromotingcoagulation.Asastrongchemoattractantforneutrophilsandfibroblasts,PF4probablyhasaroleininflammationandwoundrepair.Oncostatin-AisamemberoftheCXCchemokinefamily.FurThermore,itisusedasaninhibitorintheangiogenesisduringtumortherapy.
Description
PlateletFactor-4(CXCL4)BovineRecombinantproducedinE.Coliisanon-glycosylatedpolypeptidechaincontaining88aminoacidandhavingamolecularmassofapproximately9.5kDa.PF4ispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutionin20mMPBand500mMNaCl,pH7.0.
Solubility
ItisrecommendedtoreconstitutethelyophilizedPlateletFactor-4insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedCXCL4althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionPlateletFactor-4shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
ESSFPATFVPLPADSEGGEDEDLQCVCLKTTSGINPRHISSLEVIGAGTHCPSPQLLATKKTGRKICLDQQRPLYKKILKKLLDGDES.
BIOLOGicalActivity
Thebiologicalactivitydeterminedbyachemotaxisbioassayusinghumanneutrophilsis10-100ng/ml.
SafetyDataSheet
SDS