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Prospec/MIG Bovine/MIG (CXCL9) Bovine Recombinant/100µg/CHM-041-100µg
Cataloguenumber
CHM-041
Synonyms
SmallinducIBLecytokineB9,CXCL9,GammaINF-inducedmonokine,MIG,chemokine(C-X-Cmotif)ligand9,CMK,Humig,SCYB9,crg-10,monokineinducedbygamma-INF.
Introduction
Chemokine(C-X-Cmotif)ligand9(CXCL9)isasmallcytokinebelongstotheCXCchemokinefamilythatisalsoknownasMonokineinducedbygammaINF(MIG).CXCL9iscloselyrelatedtotwootherCXCchemokinescalledCXCL10andCXCL11,whosegenesarelocatednearthegeneforCXCL9onhumanchromosome4.CXCL9,CXCL10andCXCL11allelicittheirchemotacticfunctionsbyinteractingwiththechemokinereceptorCXCR3.
Description
MIG(CXCL9)BovineRecombinantproducedinE.Coliisanon-glycosylatedpolypeptidechaincontaining104aminoacidsandhavingamolecularmassofapproximately18.0kDa.MIGispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutionin20mMPBand500mMNaCl,pH7.0.
Solubility
ItisrecommendedtoreconstitutethelyophilizedMIG(CXCL9)insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedMIGalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionMIG(CXCL9)shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan96.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
VPAIRNGRCSCINTSQGMIHPKSLKDLKQFAPSPSCEKTEIIATMKNGNEACLNPDLPEVKELIKEWEKQVNQKKKQRKGKKYKKTKKVPKVKRSQRPSQKKTT.
BIOLOGicalActivity
Thebiologicalactivitydeterminedbyachemotaxisbioassayusinghumanlymphocytesis0.1-1.0ng/ml.
SafetyDataSheet
SDS