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Prospec/Lymphotactin Rat/Lymphotactin (XCL1) Rat Recombinant/50µg/CHM-038-50µg
Cataloguenumber
CHM-038
Synonyms
XCL1,CytokineSCM-1,ATAC,Lymphotaxin,SCM-1-alpha,SmallinducIBLecytokineC1,XCchemokineligand1,LTN,LPTN,SCM1,SCM-1,SCYC1,SCM-1a.
Introduction
XCL1isasmallcytokinebelongstotheXCchemokinefamilythatisalsoknownaslymphotactin.XCL1isfoundinhighlevelsinspleen,thymus,intestineandperipheralbloodleukocytes,andatlowerlevelsinlung,prostateglandandovary.CellularsourcesforXCL1includeactivatedthymicandperipheralbloodCD8+Tcells.ThischemokineattractsTcells.Inhumans,XCL1iscloselyrelatedtoXCL2,whosegeneisfoundatthesamelocusonchromosome1.XCL1inducesitchemotacticfunctionbybindingtoachemokinereceptorcalledXCR1.
Description
Lymphotactin(XCL1)RatRecombinantproducedinE.Coliisanon-glycosylatedpolypeptidechaincontaining93aminoacidsandhavingamolecularmassofapproximately10.0kDa.Lymphotactinispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutioninPBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedLymphotactininsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedXCL1althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionLymphotactinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
VGTEVLQESICVSLRTQRLPVQKIKTYTIKEGAMRAVIFVTKRGLRICADPQAKWVKTAIKTVDGRASASKSKAETIPTQAQRSASTAVTLTG.
BIOLOGicalActivity
TheED50asdeterminedbyachemotaxisbioassayusinghumanXCR1transfectedmurineBaF3cells<100ng/ml,correspondingtoaspecificactivityof>1.0×104IU/mg.
SafetyDataSheet
SDS