Cataloguenumber
CHM-336
Synonyms
SmallinducIBLecytokineB10,CXCL10,10kDa,Gamma-IP10,IP-10,chemokine(C-X-Cmotif)ligand10,C7,IFI10,INP10,crg-2,mob-1,SCYB10,gIP-10.
Introduction
Chemokine(C-X-Cmotif)ligand10(CXCL10)isasmallcytokinebelongingtotheCXCchemokinefamily.CXCL10issecretedbyseveralcelltypes.Thesecelltypesincludemonocytes,endothelialcellsandfibroblasts.CXCL10hasbeenattributedtoseveralroles,suchaschemoattractionformonocytesandTcells,promotionofTcelladhesiontoendothelialcells,antitumoractivity,andinhibitionofbonemarrowcolonyformationandangiogenesis.ThegeneforCXCL10islocatedonhumanchromosome4inaclusteramongseveralotherCXCchemokines.ThischemokineelicitsitseffectsbybindingtothecellsurfacechemokinereceptorCXCR3.Thethree-dimensionalcrystalstructureofthischemokinehasbeendeterminedunder3differentconditionstoaresolutionofupto1.92A.
Description
IP-10MouseRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining77aminoacidsandhavingamolecularmassof8701Dalton.TheIP-10ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
IP-10waslyophilizedfroma0.2µmfilteredconcentratedsolutionin1×PBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIP-10insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedIP-10althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionCXCL10shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
IPLARTVRCNCIHIDDGPVRMRAIGKLEIIPASLSCPRVEIIATMKKNDEQRCLNPESKTIKNLMKAFSQKRSKRAP.
DeterminedbyitsabilitytochemoattracthCXCR3/HEK293cellsusingaconcentrationrangeof100.0-500.0ng/ml.
Proteincontent
Proteinquantitationwascarriedoutbytwoindependentmethods:1.UVspectroscopyat280nmusingtheabsorbencyvalueof0.02astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).2.AnalysisbyRP-HPLC,usingastandardsolutionofIP-10asaReferenceStandard.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.