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Prospec/GCP2 Bovine/Granulocyte Chemotactic Protein 2 (CXCL6) Bovine Recombinant/100µg/CHM-040-100µg
Cataloguenumber
CHM-040
Synonyms
C-X-Cmotifchemokine6,Chemokinealpha3,CKA-3,Granulocytechemotacticprotein2,GCP-2,Small-inducIBLecytokineB6.
Introduction
GranulocyteChemotacticProtein2(CXCL6),alsoknownasGCP-2,isaChemotacticforneutrophilgranulocytes.GCP-2signalsthroughbindingandactivationofitsreceptors(CXCR1andCXCR2).GCP-2hasstrongantibacterialactivityagainstGram-positiveandGram-negativebacteriainadditiontoitschemotacticandangiogenicproperty.
Description
GranulocyteChemotacticProtein2(CXCL6)BovineRecombinantproducedinE.Coliisanon-glycosylatedpolypeptidechaincontaining76aminoacidsandhavingamolecularmassofapproximately8.0kDa.GCP2ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutionin20mMPBand500mMNaCl,pH7.0.
Solubility
ItisrecommendedtoreconstitutethelyophilizedGranulocyteChemotacticProtein2(CXCL6)insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedGCP2althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionGranulocyteChemotacticProtein2(CXCL6)shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
GPVAAVVRELRCVCLTTTPGIHPKTVSDLQVIAAGPQCSKVEVIATLKNGREVCLDPEAPLIKKIVQKILDSGKNN.
BIOLOGicalActivity
Thebiologicalactivitydeterminedbyachemotaxisbioassayusinghumanneutrophilsis10-50ng/ml.
SafetyDataSheet
SDS