Cataloguenumber
CYT-1040
Synonyms
D17Wsu104e,Il25,Ly6elg,MYDGF,Interleukin-25,IL-25,Stromalcell-derivedgrowthfactorSF20.
Introduction
Myeloid-derivedgrowthfactor(Mydgf)isaparacrine-actingproteinandabonemarrow-derivedmonocytewhichstimulatescardiacmyocytesurvivalandadaptiveangiogenesisforcardiacprotectionandrepairaftermyocardialinfarction.MydgfinducesendothelialcellproliferationthroughaMAPK1/3-,STAT3-andCCND1-mediatedsignalinglane.Whencomparingwild-typemicetomicewithaMydgf-deficiency,thelaterdeveloplargerinfarctscarsandmoreacutecontractiledysfunction.
Description
MYDGFMouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining165aminoacids(25-166a.a)andhavingamolecularmassof18.1kDa.
MYDGFisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
MYDGFproteinsolution(0.5mg/ml)containing20mMTris-HCl(pH8.0),0.1MNaCland10%glycerol.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSVSEPTTVPFDVRPGGVVHSFSQDVGPGNKFTCTFTYASQGGTNEQWQMSLGTSEDSQHFTCTIWRPQGKSYLYFTQFKAELRGAEIEYAMAYSKAAFERESDVPLKSEEFEVTKTAVSHRPGAFKAELSKLVIVAKAARSEL.
SafetyDataSheet
SDS